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Enhanced accumulation of betulinic acid in transgenic hairy roots of Senna obtusifolia growing in the Sprinkle Bioreactor and evaluation of their biological properties in various biological models

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Enhanced accumulation of betulinic acid in transgenic hairy roots of Senna obtusifolia growing in the Sprinkle Bioreactor and evaluation of their biological properties in various biological models

dc.contributor.author	Kowalczyk, Tomasz
dc.contributor.author	Sitarek, Przemysław
dc.contributor.author	Toma, Monika
dc.contributor.author	Domínguez Martín, Eva María
dc.contributor.author	Śliwiński, Tomasz
dc.contributor.author	Falcó Ferrando, Irene Lourdes
dc.contributor.author	Rijo, Patricia
dc.date.accessioned	2023-05-25T14:26:58Z
dc.date.available	2023-05-25T14:26:58Z
dc.date.issued	2021
dc.identifier.citation	Kowalczyk, Tomasz Sitarek, Przemys&#322aw Toma, Monika Domínguez Martín, Eva María &#346liwi&#324ski, Tomasz Falcó Ferrando, Irene Lourdes Rijo, Patricia 2021 Enhanced accumulation of betulinic acid in transgenic hairy roots of Senna obtusifolia growing in the Sprinkle Bioreactor and evaluation of their biological properties in various biological models Chemistry & Biodiversity 18 8
dc.identifier.uri	https://hdl.handle.net/10550/86951
dc.description.abstract	Betulinic acid, which is found in transgenic roots of Senna obtusifolia (L.) H.S.Irwin & Barneby, is a pentacyclic triterpene with distinctive pharmacological activities. In this study, we report the differences in the content of betulinic acid and selected anthraquinones in transgenic S. obtusifolia hairy roots with overexpression of the PgSS1 gene (SOPSS2 line) and in transformed hairy roots without this genetic construct (SOA41 line). Both hairy root lines grew in 10 L sprinkle bioreactor. Additionally, the extracts obtained from this plant material were used for biological tests. Our results demonstrated that the SOPSS2 hairy root cultures from the bioreactor showed an increase in the content of betulinic acid (38.125 mg/g DW), compared to the SOA41 hairy root line (4.213 mg/g DW). Biological studies have shown a cytotoxic and antiproliferative effect on U-87MG glioblastoma cells, and altering the level of apoptotic proteins (Bax, p53, Puma and Noxa). Antimicrobial properties were demonstrated for both tested extracts, with a stronger effect of SOPSS2 extract. Moreover, both extracts showed moderate antiviral properties on norovirus surrogates.
dc.relation.ispartof	Chemistry & Biodiversity, 2021, vol. 18, num. 8
dc.subject	Biodiversitat
dc.title	Enhanced accumulation of betulinic acid in transgenic hairy roots of Senna obtusifolia growing in the Sprinkle Bioreactor and evaluation of their biological properties in various biological models
dc.type	journal article
dc.date.updated	2023-05-25T14:26:58Z
dc.identifier.doi	10.1002/cbdv.202100455
dc.identifier.idgrec	159269
dc.rights.accessRights	open access