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Rapid Selective Detection of Potentially Infectious Porcine Epidemic Diarrhea Coronavirus Exposed to Heat Treatments Using Viability RT-qPCR.

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Rapid Selective Detection of Potentially Infectious Porcine Epidemic Diarrhea Coronavirus Exposed to Heat Treatments Using Viability RT-qPCR.

dc.contributor.author	Falcó Ferrando, Irene Lourdes
dc.contributor.author	Randazzo, Walter
dc.contributor.author	Puente, Héctor
dc.contributor.author	Carvajal, Ana
dc.contributor.author	Sánchez, Gloria
dc.date.accessioned	2023-05-26T10:07:40Z
dc.date.available	2023-05-26T10:07:40Z
dc.date.issued	2020
dc.identifier.citation	Falcó Ferrando, Irene Lourdes Randazzo, Walter Puente, Héctor Carvajal, Ana Sánchez, Gloria 2020 Rapid Selective Detection of Potentially Infectious Porcine Epidemic Diarrhea Coronavirus Exposed to Heat Treatments Using Viability RT-qPCR. Frontiers In Microbiology 11 1911 1 8
dc.identifier.uri	https://hdl.handle.net/10550/86969
dc.description.abstract	Coronaviruses (CoVs) cause severe respiratory, enteric, and systemic infections in a wide range of hosts, including humans and animals. Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family, is the etiological agent of porcine epidemic diarrhea (PED), a highly contagious intestinal disease affecting pigs of all ages. In this study, we optimized a viability real-time reverse transcriptase polymerase chain reaction (RT-qPCR) for the selective detection of infectious and heat-inactivated PEDV. PEMAX , EMA , and PMAxx photoactivable dyes along with PtCl4 and CDDP platinum compounds were screened as viability markers using two RT-qPCR assays: firstly, on PEDV purified RNA, and secondly on infectious and thermally inactivated virus suspensions. Furthermore, PMAxx pretreatment matched the thermal inactivation pattern obtained by cell culture better than other viability markers. Finally, we further optimized the pretreatment by coupling viability markers with Triton X-100 in inoculated serum resulting in a better estimation of PEDV infectivity than RT-qPCR alone. Our study has provided a rapid analytical tool based on viability RT-qPCR to infer PEDV infectivity with potential application for feed and feed ingredients monitoring in swine industry. This development would allow for greater accuracy in epidemiological surveys and outbreak investigations.
dc.language.iso	eng
dc.relation.ispartof	Frontiers In Microbiology, 2020, vol. 11, num. 1911, p. 1-8
dc.subject	Microbiologia
dc.title	Rapid Selective Detection of Potentially Infectious Porcine Epidemic Diarrhea Coronavirus Exposed to Heat Treatments Using Viability RT-qPCR.
dc.type	journal article
dc.date.updated	2023-05-26T10:07:40Z
dc.identifier.doi	10.3389/fmicb.2020.01911
dc.identifier.idgrec	159275
dc.rights.accessRights	open access