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An SPM-enriched marine oil supplement shifted microglia polarization toward M2 ameliorating retinal degeneration in rd10 mice

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An SPM-enriched marine oil supplement shifted microglia polarization toward M2 ameliorating retinal degeneration in rd10 mice

dc.contributor.author	Olivares González, Lorena
dc.contributor.author	Velasco Gomariz, Sheyla
dc.contributor.author	Gallego, Idoia
dc.contributor.author	Esteban Medina, Marina
dc.contributor.author	Puras, Gustavo
dc.contributor.author	Loucera, Carlos
dc.contributor.author	Martínez-Romero, Alicia
dc.contributor.author	Peña Chilet, María del Carmen
dc.contributor.author	Pedraz, José Luis
dc.contributor.author	Rodrigo Nicolás, Regina
dc.date.accessioned	2023-06-20T13:12:23Z
dc.date.available	2023-06-20T13:12:23Z
dc.date.issued	2022
dc.identifier.citation	Olivares González, Lorena elasco Gomariz, Sheyla Gallego, Idoia Esteban Medina, Marina Puras, Gustavo Loucera, Carlos Martínez Romero, Alicia Peña Chilet, María del Carmen Pedraz, José Luis Rodrigo Nicolás, Regina 2022 An SPM-enriched marine oil supplement shifted microglia polarization toward M2 ameliorating retinal degeneration in rd10 mice Antioxidants 12 1 98
dc.identifier.uri	https://hdl.handle.net/10550/88362
dc.description.abstract	Retinitis pigmentosa (RP) is the most common inherited retinal dystrophy causing progressive vision loss. It is accompanied by chronic and sustained inflammation, including M1 microglia activation. This study evaluated the effect of an essential fatty acid (EFA) supplement containing specialized pro-resolving mediators (SPMs), on retinal degeneration and microglia activation in rd10 mice, a model of RP, as well as on LPS-stimulated BV2 cells. The EFA supplement was orally administered to mice from postnatal day (P)9 to P18. At P18, the electrical activity of the retina was examined by electroretinography (ERG) and innate behavior in response to light were measured. Retinal degeneration was studied via histology including the TUNEL assay and microglia immunolabeling. Microglia polarization (M1/M2) was assessed by flow cytometry, qPCR, ELISA and histology. Redox status was analyzed by measuring antioxidant enzymes and markers of oxidative damage. Interestingly, the EFA supplement ameliorated retinal dysfunction and degeneration by improving ERG recording and sensitivity to light, and reducing photoreceptor cell loss. The EFA supplement reduced inflammation and microglia activation attenuating M1 markers as well as inducing a shift to the M2 phenotype in rd10 mouse retinas and LPS-stimulated BV2 cells. It also reduced oxidative stress markers of lipid peroxidation and carbonylation. These findings could open up new therapeutic opportunities based on resolving inflammation with oral supplementation with SPMs such as the EFA supplement.
dc.language.iso	eng
dc.relation.ispartof	Antioxidants, 2022, vol. 12, num. 1, p. 98
dc.subject	Ulls Malalties i defectes
dc.subject	Medicaments Assaigs clínics
dc.title	An SPM-enriched marine oil supplement shifted microglia polarization toward M2 ameliorating retinal degeneration in rd10 mice
dc.type	journal article
dc.date.updated	2023-06-20T13:12:23Z
dc.identifier.doi	10.3390/antiox12010098
dc.identifier.idgrec	160619
dc.rights.accessRights	open access