We conceptualize entrepreneurial ecosystems as fundamentally reliant on networks and explore how and under what conditions inter-organizational networks lead an entrepreneurial ecosystem to form and evolve. It is widely accepted that entre preneurial ecosystems possess a variety of symbiotic relationships. Research has focused considerable eforts in refning the structure and content of resources found within these networked relationships. However, merely focusing on actor-level char acterizations dilutes the notion that social relationships change and are complex. There has been little conceptual treatment of the behavioral and governance fac tors that underpin how quality interactions composing an entrepreneurial ecosystem develop and change over time. In response, we provide a longitudinal ethnographic study examining how ecosystems are managed and evolve in their relational con fgurations and governance at critical junctures. Using mixed methods and data col lected over 3 years, we reveal a cyclical process of relational development central to the initiation, development, and maintenance phases of a valuable entrepreneurial ecosystem. We contribute to a conceptualization of efective ecosystems as reliant on networks, we reveal the behavior and governance characteristics at play in the entrepreneurial ecosystem during each phase of its evolution.