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Production of bone mineral material and BMP-2 in osteoblasts cultured on double acid-etched titanium

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Production of bone mineral material and BMP-2 in osteoblasts cultured on double acid-etched titanium

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dc.contributor.author Velázquez Cayón, Rocío es
dc.contributor.author Castillo Dalí, Gabriel es
dc.contributor.author Corcuera Flores, José Ramón es
dc.contributor.author Serrera Figallo, María Ángeles es
dc.contributor.author Castillo Oyagüe, Raquel es
dc.contributor.author González Martín, Maribel es
dc.contributor.author Gutierrez Pérez,José Luis es
dc.contributor.author Torres Lagares, Daniel es
dc.date.accessioned 2023-06-22T12:12:36Z
dc.date.available 2023-06-22T12:12:36Z
dc.date.issued 2017 es
dc.identifier.citation Velázquez-Cayón R, Castillo-Dalí G, Corcuera-Flores JR, Serrera-Figallo MA, Castillo-Oyagüe R, González-Martín M, Gutierrez-Pérez JL, Torres-Lagares D. Production of bone mineral material and BMP-2 in osteoblasts cultured on double acid-etched titanium. Med Oral Patol Oral Cir Bucal. 2017 Sep 1;22 (5):e651-9. es
dc.identifier.uri https://hdl.handle.net/10550/88454
dc.description.abstract Background: The study of osteoblasts and their osteogenic functions is essential in order to understand them and their applications in implantology. In this sense, this study try to study BMP-2 production and bone matrix deposition, in addition to other biological variables, in osteoblasts cultured on a rough double acid-etched titanium surface (Osseotite®, Biomet 3i, Palm Beach Garden, Florida, USA) in comparison to a smooth titanium surface (machined) and a control Petri dish. Material and Methods: An in vitro prospective study. NHOst human osteoblasts from the femur were cultured on three different surfaces: Control group: 25-mm methacrylate dish (n = 6); Machined group: titanium discs with machined surface (n = 6) and Experimental group: titanium discs with a double acid-etched nitric and hydrofluoric Osseotite® acid surface (n = 6). A quantification of the mitochondrial membrane potential, and studies of apoptosis, mobility and adhesion, bone productivity (BMP-2) and cellular bone synthesis were carried out after culturing the three groups for forty-eight hours. Results: A statistically significant difference was observed in the production of BMP-2 between the experimental group and the other two groups (22.33% ± 11.06 vs. 13.10% ± 5.51 in the machined group and 3.88% ± 3.43 in the control group). Differences in cellular bone synthesis were also observed between the groups (28.34% ± 14.4% in the experimental group vs. 20.03% ± 6.79 in the machined group and 19.34% ± 15.93% in the control group). Conclusions: In comparison with machined surfaces, Osseotite® surfaces favor BMP-2 production and bone synthesis as a result of the osteoblasts in contact with it. Key words:BMP-2, Cytoskeleton, cell culture, bone matrix, apoptosis, cell viability. es
dc.title Production of bone mineral material and BMP-2 in osteoblasts cultured on double acid-etched titanium es
dc.type journal article es_ES
dc.subject.unesco UNESCO:CIENCIAS MÉDICAS es
dc.identifier.doi 10.4317/medoral.22071 es
dc.type.hasVersion VoR es_ES

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