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Design, Calibration, and Application of a Robust, Cost-Effective, and High-Resolution Lensless Holographic Microscope

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Design, Calibration, and Application of a Robust, Cost-Effective, and High-Resolution Lensless Holographic Microscope

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dc.contributor.author	Micó Serrano, Vicente
dc.contributor.author	Picazo Bueno, José Ángel
dc.contributor.author	Trindade, Karina
dc.contributor.author	Sanz Sabater, Martin
dc.date.accessioned	2023-06-23T10:10:19Z
dc.date.available	2023-06-23T10:10:19Z
dc.date.issued	2022
dc.identifier.citation	Micó Serrano, Vicente Picazo Bueno, José Ángel Trindade, Karina Sanz Sabater, Martin 2022 Design, Calibration, and Application of a Robust, Cost-Effective, and High-Resolution Lensless Holographic Microscope Sensors 22 2 553 570
dc.identifier.uri	https://hdl.handle.net/10550/88527
dc.description.abstract	Lensless holographic microscope (LHM) is an emerging very promising technology that provides high-quality imaging and analysis of biological samples without utilizing any lens for imaging. Due to its small size and reduced price, LHM can be a very useful tool for the point-of-care diagnosis of diseases, sperm assessment, or microfluidics, among others, not only employed in advanced laboratories but also in poor and/or remote areas. Recently, several LHMs have been reported in the literature. However, complete characterization of their optical parameters remains not much presented yet. Hence, we present a complete analysis of the performance of a compact, reduced cost, and high-resolution LHM. In particular, optical parameters such as lateral and axial resolutions, lateral magnification, and field of view are discussed into detail, comparing the experimental results with the expected theoretical values for different layout configurations. We use high-resolution amplitude and phase test targets and several microbeads to characterize the proposed microscope. This characterization is used to define a balanced and matched setup showing a good compromise between the involved parameters. Finally, such a microscope is utilized for visualization of static, as well as dynamic biosamples.
dc.language.iso	eng
dc.relation.ispartof	Sensors, 2022, vol. 22, num. 2, p. 553-570
dc.subject	Òptica
dc.subject	Optometria
dc.title	Design, Calibration, and Application of a Robust, Cost-Effective, and High-Resolution Lensless Holographic Microscope
dc.type	journal article
dc.date.updated	2023-06-23T10:10:20Z
dc.identifier.doi	10.3390/s22020553
dc.identifier.idgrec	160531
dc.rights.accessRights	open access