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Combined dynamics of the 500-600 nm leaf absorption and chlorophyll fluorescence changes in vivo: Evidence for the multifunctional energy quenching role of xanthophylls

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Combined dynamics of the 500-600 nm leaf absorption and chlorophyll fluorescence changes in vivo: Evidence for the multifunctional energy quenching role of xanthophylls

dc.contributor.author	Van Wittenberghe, Shari
dc.contributor.author	Laparra Pérez-Muelas, Valero
dc.contributor.author	García Plazaola, José Ignacio
dc.contributor.author	Fernandez Marín, Beatriz
dc.contributor.author	Porcar Castell, Albert
dc.contributor.author	Moreno, José
dc.date.accessioned	2023-06-28T16:12:25Z
dc.date.available	2023-06-28T16:12:25Z
dc.date.issued	2021
dc.identifier.citation	Van Wittenberghe, Shari Laparra Pérez-Muelas, Valero García Plazaola, José Ignacio Fernandez Marín, Beatriz Porcar Castell, Albert Moreno, José 2021 Combined dynamics of the 500-600 nm leaf absorption and chlorophyll fluorescence changes in vivo: Evidence for the multifunctional energy quenching role of xanthophylls Biochimica et Biophysica Acta-Bioenergetics 1862 2 148351
dc.identifier.uri	https://hdl.handle.net/10550/88577
dc.description.abstract	Carotenoids (Cars) regulate the energy flow towards the reaction centres in a versatile way whereby the switch between energy harvesting and dissipation is strongly modulated by the operation of the xanthophyll cycles. However, the cascade of molecular mechanisms during the change from light harvesting to energy dissipation remains spectrally poorly understood. By characterizing the in vivo absorbance changes (ΔA) of leaves from four species in the 500-600 nm range through a Gaussian decomposition, while measuring passively simultaneous Chla fluorescence (F) changes, we present a direct observation of the quick antenna adjustments during a 3-min dark-to-high-light induction. Underlying spectral behaviours of the 500-600 nm ΔA feature can be characterized by a minimum set of three Gaussians distinguishing very quick dynamics during the first minute. Our results show the parallel trend of two Gaussian components and the prompt Chla F quenching. Further, we observe similar quick kinetics between the relative behaviour of these components and the in vivo formations of antheraxanthin (Ant) and zeaxanthin (Zea), in parallel with the dynamic quenching of singlet excited chlorophyll a (1Chla*) states. After these simultaneous quick kinetical behaviours of ΔA and F during the first minute, the 500-600 nm feature continues to increase, indicating a further enhanced absorption driven by the centrally located Gaussian until 3 min after sudden light exposure. Observing these precise underlying kinetic trends of the spectral behaviour in the 500-600 nm region shows the large potential of in vivo leaf spectroscopy to bring new insights on the quick redistribution and relaxation of excitation energy, indicating a key role for both Ant and Zea.
dc.language.iso	eng
dc.relation.ispartof	Biochimica et Biophysica Acta-Bioenergetics, 2021, vol. 1862, num. 2, p. 148351
dc.subject	Ciències de la terra
dc.title	Combined dynamics of the 500-600 nm leaf absorption and chlorophyll fluorescence changes in vivo: Evidence for the multifunctional energy quenching role of xanthophylls
dc.type	journal article
dc.date.updated	2023-06-28T16:12:26Z
dc.identifier.doi	10.1016/j.bbabio.2020.148351
dc.identifier.idgrec	142070
dc.rights.accessRights	open access