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Practical guide for setting up a Fourier light-field microscope, Appl. Opt. 62, 4228 (2023)

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Practical guide for setting up a Fourier light-field microscope, Appl. Opt. 62, 4228 (2023)

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Yun, Hui; Saavedra Tortosa, Genaro; García Sucerquia, Jorge Iván; Tolosa Ruiz, Ángel; Martínez Corral, Manuel; Sanchez Ortiga, Emilio

Aquest document és un/a article, creat/da en: 2023

Apractical guide for the easy implementation of a Fourier light-field microscope is reported. The Fourier light-field concept applied to microscopy allows the capture in real time of a series of 2D orthographic images of microscopic thick dynamic samples. Such perspective images contain spatial and angular information of the light-field emitted by the sample.Afeature of this technology is the tight requirement of a double optical conjugation relationship, and also the requirement of NA matching. For these reasons, the Fourier light-field microscope being a non-complex optical system, a clear protocol on howto set up the optical elements accurately is needed. In this sense, this guide is aimed to simplify the implementation process, with an optical bench and off-the-shelf components. This will help the widespread use of this recent technology.

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