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Flow Cytometry of Oxygen and Oxygen-Related Cellular Str ess

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Flow Cytometry of Oxygen and Oxygen-Related Cellular Str ess

dc.contributor.author	Javega, Beatriz
dc.contributor.author	Herrera Martín, Guadalupe
dc.contributor.author	Martínez-Romero, Alicia
dc.contributor.author	O'Connor Blasco, José Enrique
dc.date.accessioned	2023-11-08T13:17:23Z
dc.date.available	2023-11-08T13:17:23Z
dc.date.issued	2023
dc.identifier.uri	https://hdl.handle.net/10550/91013
dc.description.abstract	Reactive oxygen species (ROS) are unstable and highly reactive molecular forms that play physiological roles in cell signaling and immune defense. However, when ROS generation is not properly balanced by antioxidant defenses, a pathological condition known as oxidative stress arises, in association with the onset and progression of many diseases and conditions, including degeneration and aging. Biomarkers of oxidative stress in biomedicine are actively investigated using different approaches, among which flow cytometry (FCM) and other single-cell, fluorescence based techniques are most frequent. FCM is an analytical method that measures light scattering and emission of multiple fluorescences by single cells or microscopic particles at a very fast rate. To assess the specific role of ROS in oxidative stress, it is essential to detect and characterize these species accurately. However, the detection and quantitation of individual intracellular ROS and parameters of oxidative stress using fluorogenic substrates and fluorescent probes are still a challenge, because of biological and methodological issues. In this review, we present and discuss a series of complementary strategies to detect ROS or to focus on other endpoints of oxidative stress. Based on our results, we propose some recommendations for proper design of cytometric studies of oxidative stress in order to prevent or minimize the limitations and experimental errors of such approaches.
dc.language.iso	eng
dc.relation.ispartof	Oxygen, 2023, vol. 3, p. 222-255
dc.source	Javega, Beatriz Herrera Martín, Guadalupe Martínez-Romero, Alicia O'Connor Blasco, José Enrique 2023 Flow Cytometry of Oxygen and Oxygen-Related Cellular Str ess Oxygen 3 222 255
dc.subject	Bioquímica
dc.subject	Biologia
dc.title	Flow Cytometry of Oxygen and Oxygen-Related Cellular Str ess
dc.type	journal article
dc.date.updated	2023-11-08T13:17:23Z
dc.identifier.doi	10.3390/oxygen3020016
dc.identifier.idgrec	161722
dc.rights.accessRights	open access