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Biochemical characterization of three heterologous lactic acid bacteria laccases from Pediococcus, Lactobacillus and Lacto-coccus genus and their potential to degrade biogenic amines using ABTS and epicatechin as mediators

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Biochemical characterization of three heterologous lactic acid bacteria laccases from Pediococcus, Lactobacillus and Lacto-coccus genus and their potential to degrade biogenic amines using ABTS and epicatechin as mediators

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dc.contributor.author	Monroy, Isaac
dc.contributor.author	Olmeda, Isidoro
dc.contributor.author	Ferrer, Sergi
dc.contributor.author	Pardo, Isabel
dc.date.accessioned	2023-11-14T11:03:30Z
dc.date.available	2023-11-15T05:45:06Z
dc.date.issued	2024	es_ES
dc.identifier.uri	https://hdl.handle.net/10550/91128
dc.description.abstract	En este trabajo se clonaron y caracterizaron tres lacasas bacterianas de las especies Pediococcus parvulus, Lacticaseibacillus paracasei y Lactococcus lactis, aisladas de vino y queso, y se evaluó su habilidad degradadora de aminas biógenas en presencia y ausencia de los mediadores ABTS (artificial) y epicatequina (natural).	es_ES
dc.language.iso	en	es_ES
dc.subject	bacterial laccases	es_ES
dc.subject	biogenic amines degradation	es_ES
dc.subject	natural mediator	es_ES
dc.subject	epicatechin	es_ES
dc.title	Biochemical characterization of three heterologous lactic acid bacteria laccases from Pediococcus, Lactobacillus and Lacto-coccus genus and their potential to degrade biogenic amines using ABTS and epicatechin as mediators	es_ES
dc.type	dataset	es_ES
dc.subject.unesco	UNESCO::CIENCIAS DE LA VIDA	es_ES
dc.description.abstractenglish	In this study, we cloned and characterized three bacterial laccases from strains of the species Pediococcus parvulus, Lacticaseibacillus paracasei and Lactococcus lactis isolated from wine and cheese, and evaluated their biogenic amine degradation abilities in the presence/absence of artificial 2,2’-azinobis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) or natural (epicatechin) mediator compounds. Although some recombinant bacterial laccases have been characterized and re-vealed as biological tools to degrade biogenic amines using or not mediators, no previous research had been undertaken on the role of natural mediators, such as phenolic substrates found in wine and some vegetable food, in the degradation of biogenic amines. The three recombinant bacterial laccases exhibited sigmoidal kinetics, similar molecular mass, and different k0.5 and specific activi-ties on ABTS. They are acidophilic with optima temperature of 28ºC, and have low thermal stabil-ity at temperatures higher than 37ºC. The three laccases were able to degrade dopamine in ab-sence of any mediator, whereas the rest of amines were not degraded. The presence of ABTS somehow improved dopamine and tyramine degradation, while the addition of epicatechin did not improve their degradation. This is the first study in which the biogenic amine-degrading effi-ciency of an artificial mediator used by laccases is compared with a natural mediator.	es_ES
dc.accrualmethod	-	es_ES
dc.embargo.terms	0 days	es_ES
dc.type.hasVersion	AO	es_ES
dc.rights.accessRights	open access	es_ES