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Determination of isoniazid and pyridoxine in plasma sample of tuberculosis patients by micellar liquid chromatography

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Determination of isoniazid and pyridoxine in plasma sample of tuberculosis patients by micellar liquid chromatography

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dc.contributor.author	Goberna Bravo, María Ángeles
dc.contributor.author	Albiol-Chiva, Jaume
dc.contributor.author	Peris Vicente, Juan
dc.contributor.author	Carda Broch, Samuel
dc.contributor.author	Esteve i Romero, Josep-Salvador
dc.date.accessioned	2023-11-21T09:28:02Z
dc.date.available	2023-11-21T09:28:02Z
dc.date.issued	2021
dc.identifier.uri	https://hdl.handle.net/10550/91281
dc.description.abstract	It is no doubt Isoniazid is a powerful tuberculosis drug, but it might give rise to Vitamin B6 (Pyridoxine) deficiency. In this case, a usual treatment is the combined administration of Isoniazid and Pyridoxine. An easy-toconduct procedure based on Micellar Liquid Chromatography has been developed to quantify Isoniazid and Pyridoxine in plasma from Tuberculosis patients. The sample was diluted in mobile phase, filtered and directly injected, thus avoiding extraction or purification steps. Both drugs were adequately resolved from the matrix and endogenous compounds using a mobile phase made up of 0.15 M sodium dodecyl sulfate - 8%(v/v) 1-butanol - 0.01 M phosphate buffer at pH 3, running at 1 mL/min through a C18 column at 25 ◦C, in less than 8 min. Detection was carried out by UV-Absorbance at 265 nm. This innovative analytical method was validated following the Food and Drug Administration Bioanalytical Method Validation Guidance for Industry (2018) with satisfactory results. The response of the drugs was linear in the range 0.1 to 15 mg/L, with r2 > 0.9990 for both compounds. Trueness and precision were 89.2-102.3% and <8.5%, respectively. Limits of detection was 0.03 mg/L and no significant carry-over effect was noticed. Finally, the analytical method was used to determine both compounds in plasma samples of Tuberculosis patients and for stability studies.
dc.language.iso	eng
dc.relation.ispartof	Microchemical Journal, 2021, vol. 167, p. 106317
dc.source	Goberna Bravo, María Ángeles, Albiol-Chiva, Jaume, Peris Vicente, Juan, Carda Broch, Samuel, Esteve i Romero, Josep-Salvador (2021). Determination of isoniazid and pyridoxine in plasma sample of tuberculosis patients by micellar liquid chromatography. Microchemical Journal, 167 106317. https://doi.org/10.1016/j.microc.2021.106317
dc.subject	química
dc.subject	química analítica
dc.title	Determination of isoniazid and pyridoxine in plasma sample of tuberculosis patients by micellar liquid chromatography
dc.type	journal article
dc.date.updated	2023-11-21T09:28:03Z
dc.identifier.doi	10.1016/j.microc.2021.106317
dc.identifier.idgrec	147327
dc.rights.accessRights	open access