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A method to determine two antibiotics prescribed to treat nosocomial infections in plasma and urine by micellar liquid chromatography

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A method to determine two antibiotics prescribed to treat nosocomial infections in plasma and urine by micellar liquid chromatography

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dc.contributor.author	Peris Vicente, Juan
dc.contributor.author	Albiol-Chiva, Jaume
dc.contributor.author	Bose, Devasish
dc.contributor.author	Durgbanshi, Abhilasha
dc.contributor.author	Carda Broch, Samuel
dc.date.accessioned	2023-11-27T14:27:49Z
dc.date.available	2023-11-27T14:27:49Z
dc.date.issued	2023
dc.identifier.issn	1570-0232
dc.identifier.uri	https://hdl.handle.net/10550/91459
dc.description.abstract	Combined prescription of the antimicrobial drugs linezolid and meropenem is a common strategy to treat multidrug-resistant nosocomial infections. We propose an innovative method to determine these two drugs in plasma and urine, based on micellar liquid chromatography. Both biological fluids were diluted in mobile phase, filtered and directly injected, without any extraction step. Using a C18 column and a mobile phase of 0.1 M sodium dodecyl sulfate - 10 % methanol, phosphate buffered at pH 3, running under isocratic mode, both antibiotics were eluted without overlapping in<15 min. Detection was by absorbance: 255 nm for linezolid and 310 nm for meropenem. The influence of sodium dodecyl sulfate and methanol concentration on retention factor was established for both drugs using an interpretative approach assisted by chemometrics. The procedure was successfully validated following the guidelines of 2018 Bioanalytical Method Validation Guidance for Industry in terms of: linearity (determination coefficients over 0.99990), calibration range (1 - 50 mg/L), instrumental and method sensitivity, trueness (bias of −10.8 to + 2.4%), precision (relative standard deviation of < 10.2%), dilution integrity, carry-over effect, robustness and stability. It should be emphasized that the method uses low volumes of toxic and volatile solvents and can be achieved in a short period. The procedure was found useful for routine analysis, as it was cost-affordable, more eco-friendly and safer than hydroorganic HPLC, easy-to-handle and highly sample-throughput. Finally, it was applied to incurred samples of patients taking this medication.
dc.language.iso	eng
dc.relation.ispartof	Journal Of Chromatography B-Analytical Technologies In The Biomedical And Life Sciences, 2023, vol. 1225, p. 123777
dc.source	Peris Vicente, Juan, Albiol-Chiva, Jaume, Bose, Devasish, Durgbanshi, Abhilasha, Carda Broch, Samuel (202). A method to determine two antibiotics prescribed to treat nosocomial infections in plasma and urine by micellar liquid chromatography. Journal Of Chromatography B-Analytical Technologies In The Biomedical And Life Sciences, 1225, 123777. https://doi.org/10.1016/j.jchromb.2023.123777
dc.subject	química analítica
dc.subject	absorbance
dc.subject	linezolid
dc.subject	meropenem
dc.subject	nosocomial
dc.subject	validation
dc.title	A method to determine two antibiotics prescribed to treat nosocomial infections in plasma and urine by micellar liquid chromatography
dc.type	journal article
dc.date.updated	2023-11-27T14:27:49Z
dc.identifier.doi	10.1016/j.jchromb.2023.123777
dc.identifier.idgrec	161873
dc.rights.accessRights	open access