Evaluation of periimplant bone neoformation using different scanning electron microscope methods for measuring BIC.: a dog study
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Calvo Guirado, José Luis; Aguilar Salvatierra, Antonio; Guardia Muñoz, Javier; Delgado Ruiz, Rafael Arcesio; Ramírez Fernández, Piedad; Pérez Sánchez, Cristina; Gómez Moreno, Gerardo
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Aquest document és un/a article, creat/da en: 2012
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Objetives: The aim of this study was to determine which of three methods for measuring BIC (bone-to-implant
contact), using vestibular and lingual scanning electron microscopy (SEM) for different implant systems at 15, 30
and 90 days post-surgery was the most precise. An elemental analysis with SEM was used to evaluate neoformed
bone composition for three implant systems at the same study times.
Material and Methods: 36 implants were placed in eighteen Beagle dogs mandible about one year old and weighing
approximately 12-13 kg in order to evaluate bone apposition to three different implant surfaces. It was used the
third and fourth premolar and first molar distal sockets in both quadrants of the mandible (3P3, 4P4 and 1M1). Teeth
were hemi-sected and the distal roots were removed. The specimens were prepared for histological examination
and each section surface was stained using Masson's trichrome and hematoxylin and eosin stains. BIC evaluations
were performed by the three methods, BIC I (the quantity of mineralized bone in direct contact with the implant's
titanium surface across the entire threaded area); BIC II (along a line that passes from apex to apex of the implant
threads); BIC III (both in areas around and above the threads and in between threads).
Results: Both BIC and bone content were analyzed for all implants placed in P3, P4 y M1 alveoli on both, the buccal
and palatine sides (elemental analysis quantified Ca, P, O and C). It was seen it was only at the ninety-day mark
that high percentages of calcium were present.
Conclusions: This study suggest that BIC III evaluation is the most certain method for establishing the quantity of
bone formed as the BIC area.
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