All-trans retinoic acid restores gap junctional intercellular communication between oral cancer cells with upregulation of Cx32 and Cx43 expressions in vitro
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Wang, Juan; Dai, Yaohui; Huang, Yulei; Chen, Xiaohua; Wang, Hong; Hong, Yun; Xia, Yu-Juan; Cheng, Bin
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Aquest document és un/a article, creat/da en: 2013
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Objective: All-trans retinoic acid (ATRA) has been demonstrated to inhibit tumor growth by restoration of gap
junctional intercellular communication (GJIC) via upregulation of connexin (Cx) expression in some solid tumors.
However, the relationship between ATRA and GJIC remains unclear in oral squamous cell carcinoma (OSCC).
The aim of this study was to investigate the effect of ATRA on the GJIC function of OSCC.
Study design: We measured the effects of ATRA on the viability and cell cycle distribution of SCC9 and Tca8113
OSCC cells. The GJIC function was observed using the scrape-loading dye transfer technique, and the mRNA and
protein levels of Cx32 and Cx43 were detected by qRT-PCR, Western blot, and immunofluorescence assays.
Results: ATRA inhibited the growth of OSCC cells in a dose- and time-dependent manner (P <0.05) and caused
cell cycle arrest. ATRA-treated cells showed a 2.69-fold and 2.06-fold enhancement of GJIC in SCC9 and Tca8113
cells, respectively (P <0.05). Moreover, ATRA induced upregulation of Cx32 and Cx43 at both the mRNA and
protein levels in OSCC cells.
Conclusion: Our results indicated that restoration of GJIC via enhanced Cx32 and Cx43 expression might serve as
a novel mechanism for the anti-tumor effect of ATRA in OSCC.
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