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All-trans retinoic acid restores gap junctional intercellular communication between oral cancer cells with upregulation of Cx32 and Cx43 expressions in vitro

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All-trans retinoic acid restores gap junctional intercellular communication between oral cancer cells with upregulation of Cx32 and Cx43 expressions in vitro

dc.contributor.author	Wang, Juan	es
dc.contributor.author	Dai, Yaohui	es
dc.contributor.author	Huang, Yulei	es
dc.contributor.author	Chen, Xiaohua	es
dc.contributor.author	Wang, Hong	es
dc.contributor.author	Hong, Yun	es
dc.contributor.author	Xia, Yu-Juan	es
dc.contributor.author	Cheng, Bin	es
dc.date.accessioned	2014-05-29T07:11:36Z
dc.date.available	2014-05-29T07:11:36Z
dc.date.issued	2013	es
dc.identifier.citation	Wang, Juan ; Dai, Yaohui ; Huang, Yulei ; Chen, Xiaohua ; Wang, Hong ; Hong, Yun ; Xia, Yu-Juan ; Cheng, Bin. All-trans retinoic acid restores gap junctional intercellular communication between oral cancer cells with upregulation of Cx32 and Cx43 expressions in vitro. En: Medicina oral, patología oral y cirugía bucal. Ed inglesa, 2013, Vol. 18, No. 4: 569-577	es
dc.identifier.uri	http://hdl.handle.net/10550/35658
dc.description.abstract	Objective: All-trans retinoic acid (ATRA) has been demonstrated to inhibit tumor growth by restoration of gap junctional intercellular communication (GJIC) via upregulation of connexin (Cx) expression in some solid tumors. However, the relationship between ATRA and GJIC remains unclear in oral squamous cell carcinoma (OSCC). The aim of this study was to investigate the effect of ATRA on the GJIC function of OSCC. Study design: We measured the effects of ATRA on the viability and cell cycle distribution of SCC9 and Tca8113 OSCC cells. The GJIC function was observed using the scrape-loading dye transfer technique, and the mRNA and protein levels of Cx32 and Cx43 were detected by qRT-PCR, Western blot, and immunofluorescence assays. Results: ATRA inhibited the growth of OSCC cells in a dose- and time-dependent manner (P <0.05) and caused cell cycle arrest. ATRA-treated cells showed a 2.69-fold and 2.06-fold enhancement of GJIC in SCC9 and Tca8113 cells, respectively (P <0.05). Moreover, ATRA induced upregulation of Cx32 and Cx43 at both the mRNA and protein levels in OSCC cells. Conclusion: Our results indicated that restoration of GJIC via enhanced Cx32 and Cx43 expression might serve as a novel mechanism for the anti-tumor effect of ATRA in OSCC.	en_US
dc.subject	Odontología	es
dc.subject	Ciencias de la salud	es
dc.title	All-trans retinoic acid restores gap junctional intercellular communication between oral cancer cells with upregulation of Cx32 and Cx43 expressions in vitro	es
dc.type	journal article	es_ES
dc.subject.unesco	UNESCO::CIENCIAS MÉDICAS	es
dc.type.hasVersion	VoR	es_ES