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A bimolecular multicellular complementation system for the detection of syncytium formation: a new methodology for the identification of nipah virus entry inhibitors

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A bimolecular multicellular complementation system for the detection of syncytium formation: a new methodology for the identification of nipah virus entry inhibitors

dc.contributor.author	García Murria, María Jesús
dc.contributor.author	Expósito-Domínguez, Neus
dc.contributor.author	Duart, Gerard
dc.contributor.author	Mingarro Muñoz, Ismael
dc.contributor.author	Martínez Gil, Luis
dc.date.accessioned	2019-09-25T13:11:07Z
dc.date.available	2019-09-25T13:11:07Z
dc.date.issued	2019
dc.identifier.citation	García Murria, María Jesús Expósito-Domínguez, Neus Duart, Gerard Mingarro Muñoz, Ismael Martínez Gil, Luis 2019 A Bimolecular Multicellular Complementation System for the Detection of Syncytium Formation: A New Methodology for the Identification of Nipah Virus Entry Inhibitors Viruses 11 229
dc.identifier.uri	https://hdl.handle.net/10550/71572
dc.description.abstract	Fusion of viral and cellular membranes is a key step during the viral life cycle. Enveloped viruses trigger this process by means of specialized viral proteins expressed on their surface, the so-called viral fusion proteins. There are multiple assays to analyze the viral entry including those that focus on the cell-cell fusion induced by some viral proteins. These methods often rely on the identification of multinucleated cells (syncytium) as a result of cell membrane fusions. In this manuscript, we describe a novel methodology for the study of cell-cell fusion. Our approach, named Bimolecular Multicellular Complementation (BiMuC), provides an adjustable platform to qualitatively and quantitatively investigate the formation of a syncytium. Furthermore, we demonstrated that our procedure meets the requirements of a drug discovery approach and performed a proof of concept small molecule high-throughput screening to identify compounds that could block the entry of the emerging Nipah virus.
dc.language.iso	eng
dc.relation.ispartof	Viruses, 2019, vol. 11, num. 229
dc.subject	virus
dc.subject	biomolècules
dc.title	A bimolecular multicellular complementation system for the detection of syncytium formation: a new methodology for the identification of nipah virus entry inhibitors
dc.type	journal article	es_ES
dc.date.updated	2019-09-25T13:11:08Z
dc.identifier.doi	10.3390/v11030229
dc.identifier.idgrec	130374
dc.rights.accessRights	open access	es_ES