Objective. To study the effect of human plasma from different sources such as umbilical cord blood and adult blood platelet-rich plasma (PRP) on the regeneration of endometrial damage. Design. Composition analysis, in vitro approaches and a pre-clinical murine model using plasma to promote endometrial regeneration. Setting. Hospital and university laboratories. Patients/Animals. Adult plasma from 4 Atrophy patients and one fertile woman, commercial umbilical cord plasma and uterine-damaged NOD/SCID mice model were used. Intervention(s). Endometrial stromal cells from primary culture and an endometrial stem cell line were cultured in vitro and uterine-damaged NOD/SCID mice were treated with plasma samples from several origins. Main Outcome Measure(s). All plasma samples contain molecules with a high potential for regeneration (SCF, PDGFBB, THBS1, VWF). Furthermore, the highest increase in in vitro proliferation and migration rate was found when endometrial stromal cells were treated with umbilical cord plasma, adult PRP also revealed a significant increment. In the mouse model, a higher expression of Ki67 and Hoxa10 in the endometrium was detected after applying adult PRP and the proteomic analysis revealed a specific protein expression profile depending on the treatment. The damaged uterine tissue showed more pro22 regenerative markers after applying umbilical cord plasma (Stat5a, Uba3, Thy1) in comparison to the other treatments (non-activated umbilical cord plasma, activated adult PRP and not treatment). Conclusion. Human PRP possesses regeneration properties usable for endometrial pathologies. Besides that, these regenerative effects seem to be more apparent when the source of obtaining is umbilical cord blood.