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Comparison of different sources of platelet-rich plasma as treatment option for infertility-causing endometrial pathologies

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Comparison of different sources of platelet-rich plasma as treatment option for infertility-causing endometrial pathologies

dc.contributor.author	De Miguel, Lucía
dc.contributor.author	López Martínez, Sara
dc.contributor.author	Campo, Hannes
dc.contributor.author	Frances Herrero, Emilio
dc.contributor.author	Faus, Amparo
dc.contributor.author	Diaz, Ana
dc.contributor.author	Pellicer Martínez, Antonio
dc.contributor.author	Dominguez, Francisco
dc.contributor.author	Cervelló Alcaraz, Irene
dc.date.accessioned	2020-10-27T11:38:55Z
dc.date.available	2020-10-27T11:38:55Z
dc.date.issued	2020
dc.identifier.citation	De Miguel, Lucía López Martínez, Sara Campo, Hannes Frances Herrero, Emilio Faus, Amparo Diaz, Ana Pellicer Martínez, Antonio Dominguez, Francisco Cervelló Alcaraz, Irene 2020 Comparison of different sources of platelet-rich plasma as treatment option for infertility-causing endometrial pathologies Fertility and Sterility
dc.identifier.uri	https://hdl.handle.net/10550/76143
dc.description.abstract	Objective. To study the effect of human plasma from different sources such as umbilical cord blood and adult blood platelet-rich plasma (PRP) on the regeneration of endometrial damage. Design. Composition analysis, in vitro approaches and a pre-clinical murine model using plasma to promote endometrial regeneration. Setting. Hospital and university laboratories. Patients/Animals. Adult plasma from 4 Atrophy patients and one fertile woman, commercial umbilical cord plasma and uterine-damaged NOD/SCID mice model were used. Intervention(s). Endometrial stromal cells from primary culture and an endometrial stem cell line were cultured in vitro and uterine-damaged NOD/SCID mice were treated with plasma samples from several origins. Main Outcome Measure(s). All plasma samples contain molecules with a high potential for regeneration (SCF, PDGFBB, THBS1, VWF). Furthermore, the highest increase in in vitro proliferation and migration rate was found when endometrial stromal cells were treated with umbilical cord plasma, adult PRP also revealed a significant increment. In the mouse model, a higher expression of Ki67 and Hoxa10 in the endometrium was detected after applying adult PRP and the proteomic analysis revealed a specific protein expression profile depending on the treatment. The damaged uterine tissue showed more pro22 regenerative markers after applying umbilical cord plasma (Stat5a, Uba3, Thy1) in comparison to the other treatments (non-activated umbilical cord plasma, activated adult PRP and not treatment). Conclusion. Human PRP possesses regeneration properties usable for endometrial pathologies. Besides that, these regenerative effects seem to be more apparent when the source of obtaining is umbilical cord blood.
dc.language.iso	eng
dc.relation.ispartof	Fertility and Sterility, 2020
dc.subject	Obstetrícia
dc.title	Comparison of different sources of platelet-rich plasma as treatment option for infertility-causing endometrial pathologies
dc.type	journal article	es_ES
dc.date.updated	2020-10-27T11:38:56Z
dc.identifier.idgrec	141053
dc.rights.accessRights	open access	es_ES