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Comparison of two cloning systems for Oenococcus oeni 229M genes in E. coli and their impact on protein yield and laccase activity

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	Monroy, Isaac; Olmeda, Isidoro; Pardo, Isabel; Ferrer, Sergi
	Aquest document és un/a Comunicació/Ponència, creat/da en: 2022
	El objetivo de este trabajo fue comparar dos sistemas de clonaje en relación al porcentaje de clones con inserto y actividad enzimática usando los vectores pET28a (5369 bp) y pETNKI (5343 bp) para el clonaje del gen de la lacasa de Oenococcus oeni 229M (ENOLAB 4002) en E. coli DH5α.
	The objective of this work was to compare two cloning systems regarding the clones with insert percentage and enzymatic activity using either pET28a (5369 bp) or pETNKI (5343 bp) vectors for cloning the laccase gene of Oenococcus oeni 229M (ENOLAB 4002) in E. coli DH5α.... [Llegir més ...] [-] The objective of this work was to compare two cloning systems regarding the clones with insert percentage and enzymatic activity using either pET28a (5369 bp) or pETNKI (5343 bp) vectors for cloning the laccase gene of Oenococcus oeni 229M (ENOLAB 4002) in E. coli DH5α.
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