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Comparison of two cloning systems for Oenococcus oeni 229M genes in E. coli and their impact on protein yield and laccase activity

Repositori DSpace/Manakin

dc.contributor.author	Monroy, Isaac
dc.contributor.author	Olmeda, Isidoro
dc.contributor.author	Pardo, Isabel
dc.contributor.author	Ferrer, Sergi
dc.date.accessioned	2023-11-08T11:56:20Z
dc.date.available	2024-05-07T04:45:48Z
dc.date.issued	2022	es_ES
dc.identifier.uri	https://hdl.handle.net/10550/91004
dc.description.abstract	El objetivo de este trabajo fue comparar dos sistemas de clonaje en relación al porcentaje de clones con inserto y actividad enzimática usando los vectores pET28a (5369 bp) y pETNKI (5343 bp) para el clonaje del gen de la lacasa de Oenococcus oeni 229M (ENOLAB 4002) en E. coli DH5α.	es_ES
dc.description.sponsorship	101022293	es_ES
dc.language.iso	en	es_ES
dc.subject	laccases	es_ES
dc.subject	cloning vectors	es_ES
dc.subject	cloning systems	es_ES
dc.title	Comparison of two cloning systems for Oenococcus oeni 229M genes in E. coli and their impact on protein yield and laccase activity	es_ES
dc.type	conference output	es_ES
dc.subject.unesco	UNESCO::CIENCIAS DE LA VIDA	es_ES
dc.description.abstractenglish	The objective of this work was to compare two cloning systems regarding the clones with insert percentage and enzymatic activity using either pET28a (5369 bp) or pETNKI (5343 bp) vectors for cloning the laccase gene of Oenococcus oeni 229M (ENOLAB 4002) in E. coli DH5α.	es_ES
dc.accrualmethod	-	es_ES
dc.embargo.terms	6 months	es_ES
dc.type.hasVersion	AO	es_ES
dc.rights.accessRights	open access	es_ES